bio_io 0.1.2 · OCaml Package

An input channel for reading FASTA files.

Overview

Quick start

To get a list of records, use records or with_file_records.
To get a sequence of records, use: record_sequence or with_file_record_sequence.
To fold over records, use: fold_records or with_file_fold_records.
To iterate over records, use: iter_records or with_file_iter_records.

For some more info, check out the usage examples.

Function flavors

Most functions in this module come in a few different flavors.

Functions with with_file_ prefix
Functions with the _exn suffix
Functions that return Or_error.t

Functions that have the with_file_ prefix in their name take a filename string rather than a Fasta_in_channel.t. They are a convenient way to avoid wrapping your all your code in with_file function calls.

Functions that the _exn suffix in their name may raise exceptions, either from logic specific to this module, or from calls to other Core_kernel functions.

Functions that return Or_error.t catch all exceptions, even those not originating in the logic of this module. These functions shouldn't raise in practice. In other words, if you aren't using a function with _exn in the name, and you get an exception, then you have found a bug.

Here is an example of the different "flavors" of fold_records:

fold_records: the normal Fasta_in_channel.t accepting, Or_error.t returning flavor
fold_records_exn: takes Fasta_in_channel.t but may raise exceptions instead of returning Or_error.t
with_file_fold_records: takes a file name (string) rather than a Fasta_in_channel.t, and returns Or_error.t
with_file_fold_records_exn: takes a file name (string) rather than a Fasta_in_channel.t, and may raise exceptions

Examples

Return all records in a list

Simplest way. May raise exceptions.

let records = Fasta_in_channel.with_file_records_exn fname

A bit more involved, but you won't get exceptions. Instead, you have to handle the Or_error.t. One reason to do this is to give the user nicer error messages.

let records =
  match Fasta_in_channel.with_file_records name with
  | Error err ->
      eprintf "Problem reading records: %s\n" (Error.to_string_hum err);
      exit 1
  | Ok records -> records

Iterating over records

Use the iter functions when you need to go over each record and perform some side-effects with them.

Print sequence IDs and sequence lengths

let () =
  Fasta_in_channel.with_file_iter_records_exn "sequences.fasta"
    ~f:(fun record ->
      let open Fasta_record in
      printf "%s => %d\n" (id record) (String.length (seq record)))

Print sequence index, IDs, and sequence lengths.

This is like the last example except that we also want to print the index. The first record is 0, the 2nd is 1, etc.

let () =
  Fasta_in_channel.with_file_iteri_records_exn "sequences.fasta"
    ~f:(fun index record ->
      let open Fasta_record in
      printf "%d: %s => %d\n" (index + 1) (id record)
        (String.length (seq record)))

Folding over records

If you need to reduce all the records down to a single value, use the fold functions.

Get total length of all sequences in the file.

Watch out as this may raise exceptions...see the _exn suffix.

let total_length =
  Fasta_in_channel.with_file_fold_records_exn "sequences.fasta" ~init:0
    ~f:(fun length record ->
      let sequence = Fasta_record.seq record in
      length + String.length sequence)

Same thing, but this won't raise exceptions. You do have to handle Or_error.t to get the final value. Note that within the fold function, you get Fasta_record.t and not Fasta_record.t Or_error.t.

let total_length =
  match
    Fasta_in_channel.with_file_fold_records name ~init:0
      ~f:(fun length record ->
        length + String.length (Fasta_record.seq record))
  with
  | Error err ->
      eprintf "Problem reading records: %s\n" (Error.to_string_hum err);
      exit 1
  | Ok total_length -> total_length

Pipelines with records

Sometimes you have a "pipeline" of computations that you need to do one after the other on records. In that case, use the sequence functions.

You could use Lists too, or just don't bother writing things in a pipeline, but with Sequenecs you can have your nice pipelines without all the extra intermediate allocations.

Here's a silly example.

In this case, I'm using the exception throwing versions.

let () =
  Fasta_in_channel.with_file_exn name ~f:(fun chan ->
      Fasta_in_channel.record_sequence_exn chan
      (* Add sequence index to record description *)
      |> Sequence.mapi ~f:(fun i record ->
             let new_desc =
               match Fasta_record.desc record with
               | None -> Some (sprintf "sequence %d" i)
               | Some old_desc ->
                   Some (sprintf "%s -- sequence %d" old_desc i)
             in
             Fasta_record.with_desc new_desc record)
      (* Convert all sequence chars to lowercase *)
      |> Sequence.map ~f:(fun record ->
             let new_seq = String.lowercase (Fasta_record.seq record) in
             Fasta_record.with_seq new_seq record)
      (* Print sequences *)
      |> Sequence.iter ~f:(fun record ->
             print_endline @@ Fasta_record.serialize record))

One thing to watch out for though...if you get an exception half way through and you are running side-effecting code like we are here then part of your side effects will have occured and part of them will not have occured.

There are also Or_error.t flavors of the sequence functions. Just watch out because these you actually do have to deal with Or_error.t for each Fasta_record.t in the sequence.

As an alternative, you could use the record_sequence_exn function, but wrap that in the with_file function. That way you don't have to deal with the Or_error.t inside your pipeline. Instead you deal with it at the end.

let total_length =
  match
    Fasta_in_channel.with_file name ~f:(fun chan ->
        Fasta_in_channel.record_sequence_exn chan
        (* Blow up pipeline on second sequence. *)
        |> Sequence.mapi ~f:(fun i record ->
               if i = 1 then assert false;
               record)
        |> Sequence.fold ~init:0 ~f:(fun length record ->
               length + String.length (Fasta_record.seq record)))
  with
  | Error err ->
      eprintf "Problem in parsing pipeline: %s\n"
        (Error.to_string_hum err);
      exit 1
  | Ok total_length -> total_length

As you can see, if that fasta file has more than one sequence it will hit the assert false and blow up.

API

type t

exception Exn of string

The only exception that logic introduced in this module will raise. Functions may raise other exceptions that occur "outside" of the module, like `Sys_error` when trying to open a file that doesn't exist.

Open/Close

val create_exn : string -> t

create_exn filename returns a t or raises an exception if the call fails.

val create : string -> t Core_kernel.Or_error.t

create filename returns a t or an Error.t if the call fails

val stdin_exn : unit -> t

stdin_exn () returns a t or raises an exception if the call fails.

val stdin : unit -> t Core_kernel.Or_error.t

stdin () is like stdin_exn except that it returns an Or_error.t rather than raising exceptions on failure.

val close_exn : t -> unit

close_exn t closes the channel or raises an exception if the call fails.

val close : t -> unit Core_kernel.Or_error.t

close t is like close_exnt t except that it returns an Or_error.t rather than raising an exception on failure.

val with_file_exn : string -> f:(t -> 'a) -> 'a

with_file_exn file_name ~f executes ~f on the channel created from file_name and closes it afterwards. May raise exceptions.

val with_file : string -> f:(t -> 'a) -> 'a Core_kernel.Or_error.t

with_file file_name ~f is like with_file_exn file_name ~f except that it returns an Or_error.t rather than raising exceptions.

Utilities

val of_in_channel : Core_kernel.In_channel.t -> t

of_in_channel chan converts an In_channel.t to a t.

val to_in_channel : t -> Core_kernel.In_channel.t

to_in_channel t converts a t to an In_channel.t.

val equal : t -> t -> bool

equal t1 t2 compares two t for equality using In_channel.equal.

val peek_char : t -> char option

peek_char t "peeks" the next char in the channel, then rewinds the channel back so the next read is not affected.

val rewind : ?pos:Core_kernel.Int64.t -> t -> unit

rewind ~pos t seeks to the pos in the channel t. Default ~pos:0, which means go back to the beginning of the channel.

val input_record_exn : t -> Fasta_record.t Core_kernel.Option.t

input_record_exn t returns Some Fasta_record.t if there is any more. If there are no more records, None is returned. Exn is raised on bad input.

val input_record : 
  t ->
  Fasta_record.t Core_kernel.Option.t Core_kernel.Or_error.t

input_record t is like input_record_exn t except that it returns an Or_error.t rather than raising exceptions.

Getting records

As list

val records_exn : t -> Fasta_record.t Core_kernel.List.t

records_exn t returns a list of Fasta_record.t. May raise exceptions.

val records : t -> Fasta_record.t Core_kernel.List.t Core_kernel.Or_error.t

records t is like records_exn t except that it returns an Or_error.t and will not raise exceptions.

val with_file_records_exn : string -> Fasta_record.t Core_kernel.List.t

with_file_records_exn file_name is like records_exn t except that it takes a file name directly rather than taking a Fasta_in_channel.t. You can use this to avoid manually creating and closing an t, or to avoid wrapping the call in with_file.

val with_file_records : 
  string ->
  Fasta_record.t Core_kernel.List.t Core_kernel.Or_error.t

with_file_records file_name is like with_file_records_exn file_name except that it returns an Or_error.t rather than raising exceptions.

As sequence

These are a bit different:

* There are no with_file versions as you would have to do some fiddly things to keep the channel open, making them not so nice to use.

* Each Fasta_record.t that is yielded is wrapped in an Or_error.t. This is different from the iter, fold, and other non _exn functions in which case the entire result is wrapped in an Or_error.t, letting you ignore errors in the passed in ~f function and deal with failure once.

val record_sequence_exn : t -> Fasta_record.t Core_kernel.Sequence.t

record_sequence_exn t returns a Sequence.t of Fasta_record.t. May raise exceptions.

val record_sequence : 
  t ->
  Fasta_record.t Core_kernel.Or_error.t Core_kernel.Sequence.t

record_sequence t is like record_sequence_exn t except that instead of raising exceptions, each item of the sequence is a Fasta_record.t Or_error.t rather than an "unwrapped" Fasta_record.t. This could make things annoying to deal with. If you don't want exceptions, you could instead wrap your entire sequence processing pipeline in a call to with_file and handle the Or_error.t in that way. See the usage examples for more info.

Folding over records

val fold_records_exn : t -> init:'a -> f:('a -> Fasta_record.t -> 'a) -> 'a

fold_records_exn t ~init ~f reduces all records from a t down to a single value of type 'a. May raise exceptions.

val fold_records : 
  t ->
  init:'a ->
  f:('a -> Fasta_record.t -> 'a) ->
  'a Core_kernel.Or_error.t

fold_records t ~init ~f is like fold_records_exn t ~init ~f except that it returns 'a Or_error.t rather than 'a and will not raise exceptions.

val with_file_fold_records_exn : 
  string ->
  init:'a ->
  f:('a -> Fasta_record.t -> 'a) ->
  'a

with_file_fold_records_exn file_name ~init ~f is like fold_records_exn t ~init ~f except that it takes the name of a fasta file directly rather than a Fasta_in_channel.t.

val with_file_fold_records : 
  string ->
  init:'a ->
  f:('a -> Fasta_record.t -> 'a) ->
  'a Core_kernel.Or_error.t

with_file_fold_records file_name ~init ~f is like fold_records t ~init ~f except that it takes the name of a fasta file directly rather than a Fasta_in_channel.t.

With index

These four functions are like their fold counterparts except that the folding function f is also passed the index of the current record.

val foldi_records_exn : 
  t ->
  init:'a ->
  f:(int -> 'a -> Fasta_record.t -> 'a) ->
  'a

val foldi_records : 
  t ->
  init:'a ->
  f:(int -> 'a -> Fasta_record.t -> 'a) ->
  'a Core_kernel.Or_error.t

val with_file_foldi_records_exn : 
  string ->
  init:'a ->
  f:(int -> 'a -> Fasta_record.t -> 'a) ->
  'a

val with_file_foldi_records : 
  string ->
  init:'a ->
  f:(int -> 'a -> Fasta_record.t -> 'a) ->
  'a Core_kernel.Or_error.t

Iterating over records

The iter functions are like the fold functions except they do not take an init value and the f function returns unit insead of some other value 'a (and thus return unit rather than a value 'a.

They are mainly called for side effects.

val iter_records_exn : t -> f:(Fasta_record.t -> unit) -> unit

val iter_records : 
  t ->
  f:(Fasta_record.t -> unit) ->
  unit Core_kernel.Or_error.t

val with_file_iter_records_exn : string -> f:(Fasta_record.t -> unit) -> unit

val with_file_iter_records : 
  string ->
  f:(Fasta_record.t -> unit) ->
  unit Core_kernel.Or_error.t

With index

The iteri variants are like the iter functions except that the function f is passed in the index of the current record.

val iteri_records_exn : t -> f:(int -> Fasta_record.t -> unit) -> unit

val iteri_records : 
  t ->
  f:(int -> Fasta_record.t -> unit) ->
  unit Core_kernel.Or_error.t

val with_file_iteri_records_exn : 
  string ->
  f:(int -> Fasta_record.t -> unit) ->
  unit

val with_file_iteri_records : 
  string ->
  f:(int -> Fasta_record.t -> unit) ->
  unit Core_kernel.Or_error.t

package bio_io

Overview

Quick start

Function flavors

Examples

Return all records in a list

Iterating over records

Folding over records

Pipelines with records

API

Open/Close

Utilities

Getting records

As list

As sequence

Folding over records

With index

Iterating over records

With index